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Comparative Study
. 2003 Mar;20(3):368-72.
doi: 10.1023/a:1022691718226.

Disposition kinetics of alpha-tocopherol in apolipoprotein B knockout mice

Koichi Yokogawa  1 , Yuichiro Shima, Tomoka Hashimoto, Makoto Hiyajyo, Kaori Kadoyama, Junko Ishizki, Masaaki Nomura, Ken-ichi Miyamoto
Affiliations
Comparative Study

Disposition kinetics of alpha-tocopherol in apolipoprotein B knockout mice

Koichi Yokogawa et al. Pharm Res. 2003 Mar.

Abstract

Purpose: We examined the effects of apolipoprotein B (apoB) on the disposition kinetics of alpha-tocopherol by using apoB knockout mice.

Methods: The concentrations of alpha-tocopherol in plasma and tissues were measured by gas chromatography-mass spectrometry.

Results: In apob (-/-) mice, the endogenous levels of alpha-tocopherol in plasma and tissues (except liver) were significantly lower, and the liver concentration was significantly higher than those in wild-type mice. After single i.v. administration of alpha-tocopherol (25 mg/kg), the area under the plasma concentration-time curve (AUC) and the distribution volume at steady state were significantly decreased, whereas the total clearance of alpha-tocopherol was significantly increased in apob (-/-) vs. wild-type mice. Alpha-Tocopherol was highly distributed to the liver, compared with other tissues. After an oral administration of alpha-tocopherol (100 mg/kg), the intestinal absorption of alpha-tocopherol was very low in apoB knockout mice, as the value of AUC0-32h for apob (-/-) mice (17.7 +/- 8.3 (microg h/mL) was significantly less than that for apob (+/+) wild-type mice (96.5 +/- 15.8 microg h/mL, mean +/- SD of five experiments, p < 0.01). The biliary excretion of alpha-tocopherol was significantly greater in apob (+/-) mice than in apob (+/+) mice.

Conclusions: These results show that apoB plays a role in hepatic secretion and intestinal absorption of alpha-tocopherol.

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References

    1. J Lipid Res. 1988 Oct;29(10):1359-66 - PubMed
    1. Free Radic Biol Med. 2001 Jul 15;31(2):226-32 - PubMed
    1. J Lipid Res. 2000 Oct;41(10):1543-51 - PubMed
    1. JAMA. 1993 Aug 18;270(7):865-9 - PubMed
    1. Biochim Biophys Acta. 1987 Nov 21;922(2):199-205 - PubMed

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