Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Apr;10(2):175-81.
doi: 10.2174/0929866033479112.

An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation

Yuan Cheng  1 , Dongsheng Liu, Yanming Feng, Guozhong Jing
Affiliations

An efficient fusion expression system for protein and peptide overexpression in Escherichia coli and NMR sample preparation

Yuan Cheng et al. Protein Pept Lett. 2003 Apr.

Abstract

An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.

PubMed Disclaimer

Publication types

MeSH terms