doi: 10.1139/o75-126.
The isolation and characterization of the plasma membrane of cultured chinese hamster ovary cells
- PMID: 126793
- DOI: 10.1139/o75-126
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The isolation and characterization of the plasma membrane of cultured chinese hamster ovary cells
Can J Biochem.
1975 Aug.
Abstract
Plasma membranes were prepared from cultured Chinese hamster ovary cells utilizing a two-phase polymer system and were characterized by enzymatic and chemical assay, and by electron microscopy. The usual degree of purification of presumptive membrane markers such as Na+-K+ ATPase (ATP phosphohydrolase, EC 3.6.1.3) ranged from three-to eightfold. Gel electrophoresis in SDS revealed several polypeptides and two glycopeptides which were enriched in the plasma membrane fraction.
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