Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Apr;9(4):731-5.
doi: 10.3748/wjg.v9.i4.731.

Follow up of infection of chacma baboons with inoculum containing A and non-A genotypes of hepatitis B virus

Affiliations

Follow up of infection of chacma baboons with inoculum containing A and non-A genotypes of hepatitis B virus

Marina Baptista et al. World J Gastroenterol. 2003 Apr.

Abstract

Aim: To determine whether one genotype (A or non-A genotypes of HBV) predominated over the other during the course of HBV infection.

Methods: Four baboons were inoculated with HBV. DNA was extracted from serum obtained at monthly intervals post-inoculation for 52 weeks and HBV DNA was amplified using primers specific for the core region containing an insert characteristic of genotype A (nt 2 354-2 359, numbering from the EcoRI site). The amplicons were cloned into PCR-Script(TM) and a minimum of 15 clones per time point were sequenced in both directions.

Results: Both genotypes persisted for the entire follow-up period of 52 weeks. Genotype non-A predominated in two baboons and genotype A in one baboon. Neither genotype predominated in the fourth baboon, as shown at a 5 % level of testing.

Conclusion: No conclusions concerning the dominance of either genotype or the natural progression or replication rates of HBV could be drawn because the pattern of the genotypes found may have been caused by sampling fluctuations at the time of DNA extraction and cloning as a result of the very low viral loads in the baboon sera.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Sequence profiles of the core region (nucleotides 2340-2368 numbering from the EcoRI site) showing the insertion of 5’CGGGAC3’ (nucleotide 2354-2359) found in genotype A and its absence in genotypes non-A. Tracks G, A, T and C are labelled.
Figure 2
Figure 2
The change of genotype of hepatitis B virus at various time of post-infection which was represented as a percentage. The number of clones sequenced at each time point was showed in brackets. *ino: inoculum.

References

    1. Lanford RE, Michaels MG, Chavez D, Brasky K, Fung J, Starzl TE. Persistence of extrahepatic hepatitis B virus DNA in the absence of detectable hepatic replication in patients with baboon liver transplants. J Med Virol. 1995;46:207–212. - PMC - PubMed
    1. Starzl TE, Machioro TL, Peter GNH. Renal heterotransplantion from baboons to man: experience with 6 cases. Transplantation. 1964;2:752–776. - PMC - PubMed
    1. Starzl TE, Fung J, Tzakis A, Todo S, Demetris AJ, Marino IR, Doyle H, Zeevi A, Warty V, Michaels M. Baboon-to-human liver transplantation. Lancet. 1993;341:65–71. - PMC - PubMed
    1. Bailey LL, Nehlsen-Cannarella SL, Concepcion W, Jolley WB. Baboon-to-human cardiac xenotransplantation in a neonate. JAMA. 1985;254:3321–3329. - PubMed
    1. Barnard CN, Wolpowitz A, Losman JG. Heterotopic cardiac transplantation with a xenograft for assistance of the left heart in cardiogenic shock after cardiopulmonary bypass. S Afr Med J. 1977;52:1035–1038. - PubMed

Publication types