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Comparative Study
. 2003 Apr;9(4):851-7.
doi: 10.3748/wjg.v9.i4.851.

Ultrastructure and molecular biological changes of chronic gastritis, gastric cancer and gastric precancerous lesions: a comparative study

Goang-Yao Yin  1 , Wu-Ning Zhang, Xiao-Jing Shen, Yi Chen, Xue-Fen He
Affiliations
Comparative Study

Ultrastructure and molecular biological changes of chronic gastritis, gastric cancer and gastric precancerous lesions: a comparative study

Goang-Yao Yin et al. World J Gastroenterol. 2003 Apr.

Abstract

Aim: To carry out a comparative study on ultrastructure and molecular biological changes of chronic gastritis (CG), gastric cancer (GC) aand gastric precancerous lesions.

Methods: By the use of histochemical staining, SEM with EDAX, TEM with EDAX, image analysis technique, RIA and chemiluminescence method, gastric mucosa of 168 patients were synchronously analyzed in morphology, trace elements, DNA, cAMP, SOD, (3)H-TdR LCT and serum LPO were also done.

Results: The incidence of epithelial nucleoplasmic ratio >1, lobulated nuclei, inter-chromatin aggregation of granules, nucleolar hypertrophy, and the content of DNA, Zn, Cu in nuclei and serum LPO of each group were showed as belows: normal control group (0.0, 0.0, 6.7, 0.0, 12.6+/-2.7, 7.6+/-0.4, 58.4+/-0.3, 2.6+/-0.6), CSG group (5.7, 2.9, 7.4, 2.9, 15.2+/-3.1, 8.1+/-0.5, 58.9+/-0.5, 4.2+/-0.7), CAG group (31.3, 29.7, 45.3, 42.2, 16.5+/-3.1, 8.6+/-0.4, 59.3+/-0.5, 4.5+/-0.6), CA group (100.0, 100.0, 72.2, 50.0, 30.7+/-8.2, 8.8+/-0.3, 59.5+/-0.4, 6.8+/-1.6), ATP(++) group (61.5, 38.5, 23.1, 38.5, 23.5+/-8.9, 8.3+/-0.4, 59.1+/-0.4, 5.1+/-1.2), IM(++)+ATP(++)group (77.8, 55.5, 33.3, 44.4, 25.1+/-7.2, 8.4+/-0.5, 59.5+/-0.4, 6.5+/-1.1), IM(+++)+ATP(++) group (100.0, 100.0, 75.0, 62.5, 28.5+/-9.1, 8.9+/-0.5, 59.7+/-0.4, 7.6+/-0.7), IMII(b) group (100.0, 62.5, 75.0, 50.0, 27.3+/-10.3, 8.6+/-0.3, 59.5+/-0.4, 6.1+/-0.9); whereas the content of Zn, Cu in mitochondria and cAMP, SOD in gastric mucosa, and (3)H-TdR LCT of each group were showen as belows: normal control group (9.2+/-0.5, 58.3+/-0.3, 15.9+/-1.5, 170.5+/-6.1, 1079.7+/-227.4), CSG group (8.6+/-0.5, 57.8+/-0.3, 14.6+/-1.8, 163.3+/-5.6, 867.3+/-240.5), CAG group (8.3+/-0.4, 57.5+/-0.3, 13.4+/-1.8, 161.2+/-4.3, 800.9+/-221.8), CA group (8.9+/-0.4, 57.1+/-0.3, 10.2+/-3.9, 152.2+/-3.8, 325.7+/-186.8), ATP(++) group (9.1+/-0.4, 57.0+/-0.3, 12.4+/-1.8, 161.5+/-3.8, 642.9+/-174.3), IM(++)+ATP(++) group (8.6+/-0.4, 56.9+/-0.3, 12.0+/-2.3, 152.2+/-2.5, 326.3+/-160.3), IM(+++)+ATP(++) group (8.5+/-0.3, 56.8+/-0.2, 10.4+/-0.9, 147.4+/-2.6, 316.1+/-170.7), IMII(b) group (8.6+/-0.3, 56.9+/-0.3, 11.9+/-1.9, 150.0+/-2.8, 318.9+/-145.8), there were significant differences between groups (P<0.05-0.01).

Conclusion: There was a significant difference between CG and GC in their ultrastructure and molecular biology. Only on the condition of changes of internal environment in combination with the harmful effect of external environment, chronic atrophic gastritis can then develop into gastric cancer. Hence it might have similar epithelial cell ultrastructure and molecular biological changes in ATP(++), IMII(b) and cancer, hence there were similar patterns of occurrence, development and transformation. Recognition of this trend might help to explore problems of prevention and cure.

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Figures

Figure 1
Figure 1
Convolution shape of mucosa surface. × 320.
Figure 2
Figure 2
Degeneration, diabrosis, necrosis and exfoliation of gastric mucosa epithelial cells. × 640.
Figure 3
Figure 3
Cysts of gastric mucosa. × 64.
Figure 4
Figure 4
Atrophic and denatured glands propria in grid frame-work structure. × 320.
Figure 5
Figure 5
The surface of IM cells is thickly coated, intercellular boundaries are not clear. × 1250.
Figure 6
Figure 6
The hyperplastic cells are of different sizes and states, the active membranes are shaped like lesser tubercles. × 320.
Figure 7
Figure 7
The hyperplastic cells are of different sizes and states, the active membranes are shaped like lesser tubercles. × 640.
Figure 8
Figure 8
The cells of gastric cancer are conglobated and shaped like grapes. × 320.
Figure 9
Figure 9
There is an increase in inter chromatinic grandules densification, nucleolar grandules become thick nucleoli ex-pand with irregular margins. × 14000.
Figure 10
Figure 10
Nucleolar margination. × 7200.
Figure 11
Figure 11
Multi nucleoli. × 14000
Figure 12
Figure 12
Nucleolar division. × 14000.
Figure 13
Figure 13
Intranuclear inclusion. × 14000.
Figure 14
Figure 14
The densification of inter chromatinic grandules. × 14000.
See this image and copyright information in PMC

References

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    1. Yin GY, Zhang WN, Shen XJ, Chen Y, He XF. Ultrastructural and molecular diological changes of chronic gastritis and gastric cancer: a comparative study. Shijie Huaren Xiaohua Zazhi. 2002;10:668–672.
    1. Yin GY, Zhang WN, He XF, Chen Y, Shen XJ. Alterations of ultrastructures, trace elements, cAMP and cytoimmunity in sub-groups of chronic gastritis. Shijie Huaren Xiaohua Zazhi. 2002;10:673–676.
    1. Yin GY, Zhang WN, He XF, Chen Y, Yin YF, Shen XJ. Histocytepathological study on gastric mucosa of spleen deficiency syndromes. Zhongguo Zhongxiyi Jiehe Zazhi. 1999;19:660–663. - PubMed
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