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. 2003 Apr;41(4):1694-700.
doi: 10.1128/JCM.41.4.1694-1700.2003.

Listeria monocytogenes isolates from invasive infections: variation of sero- and genotypes during an 11-year period in Finland

Susanna Lukinmaa  1 , Maria Miettinen, Ulla-Maija Nakari, Hannu Korkeala, Anja Siitonen
Affiliations

Listeria monocytogenes isolates from invasive infections: variation of sero- and genotypes during an 11-year period in Finland

Susanna Lukinmaa et al. J Clin Microbiol. 2003 Apr.

Abstract

Listeria monocytogenes strains that were isolated from 314 human listeriosis cases in Finland during an 11-year period were analyzed by O:H serotyping and pulsed-field gel electrophoresis (PFGE). Serotyping divided the isolates into five serotypes, the most common being 1/2a (53%) and 4b (27%). During the study period, the number of cases caused by serotype 1/2a increased from 22% in 1990 to 67% in 2001, and those caused by serotype 4b decreased from 61 to 27%, respectively. PFGE with restriction enzyme AscI divided the strains into 81 PFGE genotypes; among strains of serotypes 1/2a and 4b, 49 and 18 PFGE types were seen, respectively. PFGE type 1 (serotype 1/2a) was the most prevalent single type (37 strains). Together with six other, closely related PFGE types, PFGE type 1 formed a group of 71 strains, representing 23% of all 314 strains. Strains of PFGE type 1 have also been isolated from cold smoked fish, suggesting a source of human infections caused by this type. Moreover, PFGE type 24 (serotype 1/2c) was significantly associated with gender: 5% of 180 male subjects but none of 132 female subjects (P = 0.012). An electronic database library was created from the PFGE profiles to make possible the prompt detection of new emerging profiles and the tracing of potential infection clusters in the future.

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Figures

FIG. 1.
FIG. 1.
Cases of L. monocytogenes infections by month from 1990 to 2001. The serotypes of the strains (a) and the five most common groups (G1, GT5, G11, G21, and G71) of closely related PFGE types (b) are indicated by colors. The seven mother-child pairs are indicate by numbers (1 to 7).
FIG. 1.
FIG. 1.
Cases of L. monocytogenes infections by month from 1990 to 2001. The serotypes of the strains (a) and the five most common groups (G1, GT5, G11, G21, and G71) of closely related PFGE types (b) are indicated by colors. The seven mother-child pairs are indicate by numbers (1 to 7).
FIG. 2.
FIG. 2.
Dendrogram of closely related PFGE types of the five most common groups (G1, GT5, G11, G21, and G71).
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