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. 2003 Apr;41(4):1736-7.
doi: 10.1128/JCM.41.4.1736-1737.2003.

Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry

T G M Bauters  1 , D Swinne, V Stove, H J Nelis
Affiliations

Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry

T G M Bauters et al. J Clin Microbiol. 2003 Apr.

Abstract

A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.

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Figures

FIG. 1.
FIG. 1.
Agreement between SPC (viability [black bars] and immunofluorescence labeling [gray bars]) and plate methods [white bars] for laboratory strains (n = 17). N, number of counts per milliliter in SPC or CFU per milliliter in the plate method.
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