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. 2003 Apr;41(4):1740-2.
doi: 10.1128/JCM.41.4.1740-1742.2003.

Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory

Sophie A Granier  1 , Véronique Leflon-Guibout, Fred W Goldstein, Marie-Hélène Nicolas-Chanoine
Affiliations

Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory

Sophie A Granier et al. J Clin Microbiol. 2003 Apr.

Abstract

The enterobacterial repetitive intergenic consensus 1R PCR method, which provided recognizable profiles for reference strains of the three species of Raoultella and the two genetic groups of Klebsiella oxytoca, was applied to 19 clinical isolates identified as K. oxytoca. By this method, as confirmed by species-specific gene sequencing, two Raoultella ornithinolytica and two unclassifiable K. oxytoca isolates were identified.

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Figures

FIG. 1.
FIG. 1.
ERIC-1R profiles of R. ornithinolytica, R. planticola, R. terrigena, and K. oxytoca strains. Lanes 1 and 10, molecular size markers (100-bp ladder; Amersham Biosciences, Little Chalfont, United Kingdom), with the most intense band corresponding to 800 bp; lanes 2 and 3, R. ornithinolytica ATCC 31898T and BM85.01.101, respectively; lanes 4 and 5, R. planticola ATCC 33531T and BM85.01.092, respectively; lanes 6 and 7, R. terrigena ATCC 33257T and BM85.01.095, respectively; lanes 8 and 9, K. oxytoca strains SL781 (genetic group oxy-1) and SL911 (genetic group oxy-2), respectively.
FIG. 2.
FIG. 2.
ERIC-1R profiles of 19 clinical isolates identified as K. oxytoca and reference strains of K. oxytoca. (A) Lanes 1 and 12, molecular size markers (100-bp ladder; Amersham Biosciences), with the most intense band corresponding to 800 bp; lanes 2 to 9, isolates SG245, SG246, SG247, SG248, SG249, SG250, SG251, and SG252, respectively; lanes 10 and 11, K. oxytoca strains SL781 (genetic group oxy-1) and SL911 (genetic group oxy-2), respectively. (B) Lanes 1 and 15, molecular size markers (100-bp ladder, Amersham Biosciences), with the most intense band corresponding to 800 bp; lanes 2 to 12, isolates SG254, SG255, SG258, SG259, SG261, SG264, SG265, SG266, SG269, SG271, and SG273, respectively; lanes 13 and 14, K. oxytoca strains SL781 (genetic group oxy-1) and SL911 (genetic group oxy-2), respectively.
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