New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis
- PMID: 12682177
- PMCID: PMC153867
- DOI: 10.1128/JCM.41.4.1750-1752.2003
New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis
Abstract
We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.
References
-
- Baron, R. C., M. H. Hatch, K. Kleeman, and J. N. MacCormack. 1982. Aseptic meningitis among members of a high school football team. An outbreak associated with echovirus 16 infection. JAMA 248:1724-1727. - PubMed
-
- Bourlet, T., S. Omar, F. Grattard, and B. Pozzetto. 1997. Detection of coxsackievirus B3 in intestinal tissue of orally-infected mice by a standardized RT-PCR assay. Clin. Diagn. Virol. 8:143-150. - PubMed
-
- Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159. - PubMed
-
- Colimon, R., S. Minjolle, P. Andre, C. T. de la Pintiere, A. Ruffault, C. Michelet, and F. Cartier. 1996. New types of primers (stair primers) for PCR amplification of the variable V3 region of the human immunodeficiency virus. J. Virol. Methods 58:7-19. - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
