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. 2003 Apr;41(4):1750-2.
doi: 10.1128/JCM.41.4.1750-1752.2003.

New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis

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New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis

Thomas Bourlet et al. J Clin Microbiol. 2003 Apr.

Abstract

We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.

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References

    1. Baron, R. C., M. H. Hatch, K. Kleeman, and J. N. MacCormack. 1982. Aseptic meningitis among members of a high school football team. An outbreak associated with echovirus 16 infection. JAMA 248:1724-1727. - PubMed
    1. Bourlet, T., S. Omar, F. Grattard, and B. Pozzetto. 1997. Detection of coxsackievirus B3 in intestinal tissue of orally-infected mice by a standardized RT-PCR assay. Clin. Diagn. Virol. 8:143-150. - PubMed
    1. Chapman, N. M., S. Tracy, C. J. Gauntt, and U. Fortmueller. 1990. Molecular detection and identification of enteroviruses using enzymatic amplification and nucleic acid hybridization. J. Clin. Microbiol. 28:843-850. - PMC - PubMed
    1. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159. - PubMed
    1. Colimon, R., S. Minjolle, P. Andre, C. T. de la Pintiere, A. Ruffault, C. Michelet, and F. Cartier. 1996. New types of primers (stair primers) for PCR amplification of the variable V3 region of the human immunodeficiency virus. J. Virol. Methods 58:7-19. - PubMed

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