Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci
- PMID: 12682182
- PMCID: PMC153896
- DOI: 10.1128/JCM.41.4.1766-1768.2003
Evaluation of a fluorescence-based PCR method for identification of serogroup a meningococci
Abstract
Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.
Figures
References
-
- Achtman, M. 1995. Epidemic spread and antigenic variability of Neisseria meningitidis. Trends Microbiol. 3:186-192. - PubMed
-
- Clarke, S. C., M. A. Diggle, and G. F. Edwards. 2001. Automated non-culture-based sequence typing of meningococci from body fluids. Br. J. Biomed. Sci. 58:230-234. - PubMed
-
- Claus, H., U. Vogel, M. Muhlenhoff, R. Gerardy-Schahn, and M. Frosch. 1997. Molecular divergence of the sia locus in different serogroups of Neisseria meningitidis expressing polysialic acid capsules. Mol. Gen. Genet. 257:28-34. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
