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. 2003 Apr;41(4):1785-7.
doi: 10.1128/JCM.41.4.1785-1787.2003.

BIBI, a bioinformatics bacterial identification tool

G Devulder  1 , G Perrière, F Baty, J P Flandrois
Affiliations

BIBI, a bioinformatics bacterial identification tool

G Devulder et al. J Clin Microbiol. 2003 Apr.

Abstract

BIBI was designed to automate DNA sequence analysis for bacterial identification in the clinical field. BIBI relies on the use of BLAST and CLUSTAL W programs applied to different subsets of sequences extracted from GenBank. These sequences are filtered and stored in a new database, which is adapted to bacterial identification.

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Figures

FIG. 1.
FIG. 1.
Graphical representation of the process of BIBI. The first stage in the process is the submission of the unknown strain sequence, which is stored on the server (step 1). Next, the search for similar sequences is carried out by BLAST on the database selected by the user (step 2). The BLAST results are then filtered by the BIBI program to build a FASTA file containing the similar sequences detected (step 3). These sequences are then multiply aligned with CLUSTAL W (step 4). The results file is generated from the distance matrix file created by CLUSTAL W (step 5). All files created during the whole process are accessible by FTP through links displayed on the HTML page sent to the user by BIBI.
FIG. 2.
FIG. 2.
Screenshot of BIBI results.
See this image and copyright information in PMC

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