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Review
. 2003 Apr;2(2):191-9.
doi: 10.1128/EC.2.2.191-199.2003.

Molecular genetics of pathogenic oomycetes

Sophien Kamoun  1
Affiliations
Review

Molecular genetics of pathogenic oomycetes

Sophien Kamoun. Eukaryot Cell. 2003 Apr.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Sequence conservation at the oomycete transcriptional start site. Sequences surrounding known and predicted transcriptional start sites (61, 76) were identified from 15 oomycete gene sequences deposited in GenBank (release 131.0) and aligned manually. Note that the exact position of the transcriptional start site varies depending on the gene examined but often corresponds to position 8 (C) or 9 (A). Consensus sequences were calculated at http://www.bio.cam.ac.uk/seqlogo.
FIG. 2.
FIG. 2.
Sequence conservation in Phytophthora introns. Consensus sequences for 5′ exon-intron junctions (A) and 3′ intron-exon junctions (B) were calculated based on 19 Phytophthora introns (GenBank release 113.0) at http://www.bio.cam.ac.uk/seqlogo. The intron sequences start at position 5 (A) and end at position 12 (B).
FIG. 3.
FIG. 3.
Sequence conservation around translational start codons of Phytophthora. Consensus sequences surrounding the ATG start codon (−10 to +3) were calculated based on 50 gene sequences (GenBank release 113.0) at http://www.bio.cam.ac.uk/seqlogo.
FIG. 4.
FIG. 4.
Schematic illustration of internuclear transfer of gene silencing in Phytophthora (107). Protoplast fusion of an antisense transgenically silenced strain (unshaded nuclei, unshaded cytoplasm) and a wild-type strain (shaded nuclei, shaded cytoplasm) resulted in heterokaryotic strains (mixed unshaded and shaded nuclei) that exhibit gene silencing (unshaded cytoplasm). Nuclear separation using single zoospore cultures derived from the heterokaryotic strains resulted in silenced strains (unshaded cytoplasm) independently of the presence of the transgene.
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