Differential response of genes for ferredoxin and ferredoxin:NADP+ oxidoreductase to nitrate and light in maize leaves

Abstract

In higher plants, ferredoxin (Fd) and Fd:NADP+ oxidoreductase (FNR, EC 1.18.1.2) are encoded by small multigene families, and the individuals transfer electrons to the dependent enzymes in the photosynthetic and the non-photosynthetic plastids. In maize, a C4 plant, expression of genes for the non-photosynthetic isoproteins, Fd VI and R-FNR, is responsive to nitrate in roots whereas the expression and the spatial distribution in the leaves have not been analysed. Here, we studied the expression pattern of a series of Fd and FNR genes in maize leaves in response to nitrate and light. Upon addition of nitrate, the transcripts for Fd VI and R-FNR rapidly accumulated in the leaves, whereas light did not induce accumulation. Expression of genes for the other isoproteins was not changed significantly by the nitrogen source. In the leaf, the transcripts for Fd VI and R-FNR were predominantly detected in mesophyll cells as were those for nitrate-assimilatory enzymes. Since R-FNR is an isoprotein transferring electrons from NADPH to non-photosynthetic type Fd, the redox equivalent is supplied in nitrate reduction, at least partially, via an oxidative pentose phosphate pathway, even in photosynthetic organs.