doi: 10.1128/iai.13.3.982-986.1976.
Purification of staphylococcal alpha-toxin by adsorption chromatography on glass
- PMID: 1270140
- PMCID: PMC420702
- DOI: 10.1128/iai.13.3.982-986.1976
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Purification of staphylococcal alpha-toxin by adsorption chromatography on glass
Infect Immun.
1976 Mar.
Abstract
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.
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