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. 1976 Mar;13(3):982-6.
doi: 10.1128/iai.13.3.982-986.1976.

Purification of staphylococcal alpha-toxin by adsorption chromatography on glass

P Cassidy, S Harshman

Purification of staphylococcal alpha-toxin by adsorption chromatography on glass

P Cassidy et al. Infect Immun. 1976 Mar.

Abstract

Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.

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References

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