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Comment
. 2003 Apr 29;100(9):4981-3.
doi: 10.1073/pnas.0930650100. Epub 2003 Apr 18.

Does clathrin pull the fission trigger?

Gilbert Di Paolo  1 , Pietro De Camilli
Affiliations
Comment

Does clathrin pull the fission trigger?

Gilbert Di Paolo et al. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Figure 1
Figure 1
Dynamin collars (arrows) observed in a variety of experimental systems. (A) Collared endocytic pits in nerve terminals of shibire mutants at the restrictive temperature (from ref. 30). (B–D) Dynamin collars around the stalks of endocytic buds on synaptic membranes incubated with brain cytosol, ATP, and GTPγS (from refs. and 50). (E and F) Collared tubular endocytic structures in the cortical cytoplasm BHK cells expressing clathrin antisense RNA, as shown by Iversen et al. (7) in this issue of PNAS. These structures, as well as the bud into which they terminate, were shown to be positive for dynamin immunoreactivity by immunogold. Note their similarity of these tubules to the typical dynamin coated tubule shown in D. (G) Cryo-electron microscopy images of liposomes incubated with purified nucleotide-free dynamin. Arrows indicate cross-sectioned collars, and arrowheads indicate individual dynamin oligomers. The dynamin coat is visible also on nontubular liposomes, raising the possibility that some of the density on the vesicular portions of the endocytic structure shown in E and F may be accounted for by dynamin (from ref. 36).
See this image and copyright information in PMC

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