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. 2003 May;162(5):1623-7.
doi: 10.1016/s0002-9440(10)64296-4.

Microtubule reduction in Alzheimer's disease and aging is independent of tau filament formation

Adam D Cash  1 , Gjumrakch Aliev, Sandra L Siedlak, Akihiko Nunomura, Hisashi Fujioka, Xiongwei Zhu, Arun K Raina, Harry V Vinters, Massimo Tabaton, Anne B Johnson, Manuel Paula-Barbosa, Jesus Avíla, Paul K Jones, Rudy J Castellani, Mark A Smith, George Perry
Affiliations

Microtubule reduction in Alzheimer's disease and aging is independent of tau filament formation

Adam D Cash et al. Am J Pathol. 2003 May.

Abstract

Biochemical studies show that phosphorylated tau, like that found in paired helical filaments (PHFs), does not promote microtubule assembly leading to the view that PHF formation leads to microtubule deficiency in Alzheimer's disease (AD). However, although this issue is one of the most important aspects to further understanding the cell biology of AD, no quantitative examination of microtubule diminution in AD and its relationship with PHFs has been performed. To examine this issue directly, we undertook a morphometric study of brain biopsy specimens from AD and control cases. Ultrastructural analysis of neurons was performed to compare the microtubule assembly state in neurons of diseased and control cases and to examine the effect of PHF accumulation. We found that both number and total length of microtubules were significantly and selectively reduced in pyramidal neurons from AD in comparison to control cases (P = 0.000004) but that this decrement in microtubule density was surprisingly unrelated to PHFs (P = 0.8). Further, we found a significant age-dependent decrease in microtubule density with aging in the control cases (P = 0.016). These findings suggest that reduction in microtubule assembly is not dependent on tau abnormalities of AD and aging.

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Figures

Figure 1.
Figure 1.
Microtubule density (A, C) and length (B, D) both show striking reductions for pyramidal neurons in AD when compared to controls (P = 0.000004, density; P = 0.00004, length), whereas no reduction was seen for adjacent nonpyramidal neurons (P = 0.90, density; P = 0.55, length) in the same section. Surprisingly, there was no difference of microtubule density (P = 0.80) or length (P = 0.15) dependent on the presence of PHFs.
Figure 2.
Figure 2.
Abundant microtubules were often seen in close juxtaposition to PHFs. Electron microscopic examinations of microtubules for both AD and control cases were performed in specimens preserved from biopsy fixed in 1.5% glutaraldehyde and 1% osmium tetroxide. Microtubules (arrowhead) could often be seen in close juxtaposition to PHFs (asterisk) in AD. n, nucleus. Scale bar, 250 nm.
Figure 3.
Figure 3.
There is a notable age-dependent decrease in microtubule density in pyramidal neurons among control individuals (P = 0.016; regression analysis, r2 = 0.9841). Error bars represent SEM.
See this image and copyright information in PMC

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