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. 1975 Oct;2(10):1967-74.
doi: 10.1093/nar/2.10.1967.

Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide

A Razin, D Goren, J Friedman
Free PMC article

Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide

A Razin et al. Nucleic Acids Res. 1975 Oct.
Free PMC article

Abstract

Nicotinamide was found to be a potent inhibitor of DNA methylation in vivo without interfering with protein or DNA synthesis. The inhibition of DNA methylation in a phage-infected cell resulted in a parallel decrease in the production of viable virus particles. In vitro experiments revealed that nicotinamide inhibits DNA methylase activity in a competitive fashion with respect to S-adenosylmethionine and non-competitively with respect to DNA. These results were interpreted to mean that DNA methylation is an essential step in the process of maturation of the bacteriophage phichi174.

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References

    1. J Mol Biol. 1962 Mar;4:142-60 - PubMed
    1. Biochemistry. 1972 Feb 1;11(3):393-7 - PubMed
    1. Biochem Biophys Res Commun. 1972 Jul 11;48(1):58-64 - PubMed
    1. Chemotherapy. 1972;17(4):217-58 - PubMed
    1. J Bacteriol. 1973 Jun;114(3):1143-50 - PubMed

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