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. 2003 May;9(5):1067-71.
doi: 10.3748/wjg.v9.i5.1067.

Effect of 1,25-dihydroxyvitamin D3 on preventing allograft from acute rejection following rat orthotopic liver transplantation

Ai-Bin Zhang  1 , Shu-Sen Zheng, Chang-Ku Jia, Yan Wang
Affiliations

Effect of 1,25-dihydroxyvitamin D3 on preventing allograft from acute rejection following rat orthotopic liver transplantation

Ai-Bin Zhang et al. World J Gastroenterol. 2003 May.

Abstract

Aim: To study the mechanism and the preventive role of 1,25-dihydroxyvitamin D3 in acute rejection following orthotopic liver transplantation.

Methods: Rats were randomly divided as donors or recipients for orthotopic liver allotransplantation model. Four groups were designed in the study, Group I: syngenic control (Wistar to Wistar); Group II: acute rejection (SD to Wistar); Group III: acute rejection treated with cyclosporine A, and Group IV: acute rejection treated with 1,25-(OH)2D3. Liver function, rejection activity index and mRNA of IFN-gamma, IL-10 in intragraft in recipients were measured in on day 1, 5, 7, 15, 30 posttransplant for assessing graft function, severity of acute rejection and immune state of recipients.

Results: Survival time of recipients in Group VI was significantly prolonged (over 100 days) in comparison with other groups (vs Group II, P<0.001; vs Group III, P>0.05). After treatment with 1,25-(OH)2 D3, mean value of all the assay tested on each experimental time was compared, liver function in group IV was significantly improved (AST 127+/-41 U/L-360+/-104 U/L, BIL 13+/-5 mmol/l-38+/-11 mmol/l; vs Group II, P<0.05; vs Group III, P>0.05. Rejection activity index was significantly decreased (0-3.3+/-1.6; vs Group II, P<0.05; vs Group III, P>0.05). Level of hepatic IFN-gamma mRNA in group IV was decreased, while level of hepatic IL-10 mRNA was increased (vs Group II, P<0.05; vs Group III, P>0.05).

Conclusion: Our results indicated that 1,25-(OH)2D3 induced the secretion of cytokine toward to Th2 type, which would alleviate acute rejection, protect liver function and prolong survival of recipient after orthotopic liver transplantation.

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Figures

Figure 1
Figure 1
Effect of 1,25-(OH)2 D3 on survival of rat recipients of an othtopic liver allograft (Kaplan-Meier Survival Curve). When Group III was compared with Grou II: P = 0.0005. When Group IV was compared with Grou II: P = 0.0005. When Group IV was compared with Group III: P = 0.70.
Figure 2
Figure 2
Effect of 1,25-(OH)2 D3 on graft function (-x ± s). aP < 0.05, vs Group I; bP < 0.05, vs Group II.
Figure 3
Figure 3
Effect of 1,25-(OH)2 D3 on Rejection Activity Index (RAI). (-x ± s). aP < 0.05, vs Group I; bP < 0.05 vs Group II.
Figure 4
Figure 4
Effect of 1,25-(OH)2 D3 on IFN-γ and IL-10 gene transcription (-x ± s, analyzed by RT-PCR). aP < 0.05, vs Group I; bP < 0.05 vs Group II.
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