doi: 10.1016/S0378-1097(03)00205-2.
Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation
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- PMID: 12725935
- DOI: 10.1016/S0378-1097(03)00205-2
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Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation
FEMS Microbiol Lett.
.
Abstract
A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.
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