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. 1976 Mar;3(3):599-613.
doi: 10.1093/nar/3.3.599.

Function of DNA polymerase I in RNA-primed synthesis of bacteriophage M-13 duplex DNA

P K Schneck, W L Staudenbauer, P H Hofschneider
Free PMC article

Function of DNA polymerase I in RNA-primed synthesis of bacteriophage M-13 duplex DNA

P K Schneck et al. Nucleic Acids Res. 1976 Mar.
Free PMC article

Abstract

Cell-free extracts from Escherichia coli contain a DNA polymerase activity resistant to SH-blocking agents, which is capable of synthesizing complementary strand DNA on a circular M-13 DNA template by extension of RNA primers. This activity is considered to be identical with DNA polymerase I (or some altered form of this enzyme) since it is missing in extracts from po1A- cells. DNA synthesis in the presence of SH-blocking agents occurs at a reduced rate as compared to untreated controls and leads to the formation of DNA chains of defined size (0.4-0.5 genome's length). It is concluded that efficient M-13 duplex DNA synthesis requires the cooperation of both DNA polymerase I and III.

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References

    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3679-83 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2288-91 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5 - PubMed
    1. J Biol Chem. 1973 Feb 25;248(4):1361-4 - PubMed