Switching to cue-directed behavior: specific for ventral striatal dopamine but not ventral pallidum/substantia innominata gaba as revealed by a swimming-test procedure in rats

Abstract

In this study it was investigated whether ventral striatal dopamine-induced changes in switching to cue-directed behavioral patterns were funnelled via the rostral areas of the ventral pallidum/substantia innominata (VP/SI) complex and, if so, whether changes in switching to cue-directed behavioral patterns could be elicited in the VP/SI complex by manipulating GABAergic activity. To this end rats were bilaterally equipped with cannulae directed at the ventral striatum and/or rostral VP/SI complex and subjected to a swimming-test procedure for 6 min. Injections of the dopamine-releasing agent d-amphetamine (10 microg/0.5 microl per side) enhanced the number of different cue-directed behavioral patterns while they had no effect upon the number of different non-cue-directed behavioral patterns in line with previous studies (Life Sci - 1989 1697). This increase was attenuated by a low dose of the GABAa agonist muscimol (1 ng/0.5 microl) into the rostral VP/SI complex. This dose of muscimol when injected alone into the rostral VP/SI complex had no effect upon the number of different cue-directed behavioral patterns. Only the lowest dose of the GABAa antagonist bicuculline (10-25 ng/0.5 microl per side) into the rostral VP/SI complex slightly, and in a non-d-amphetamine-like manner, increased the number of different cue-directed behavioral patterns while none of the doses had an effect on the number of different non-cue-directed behavioral patterns. Both injections of d-amphetamine into the ventral striatum and injections of bicuculline into the rostral VP/SI complex strongly increased motor activity in the 10-min period preceding the swimming test. We conclude from the data that switching to cue-directed behavioral patterns is sensitive to manipulations with the dopaminergic activity in the ventral striatum but not with the GABAergic activity in the VP/SI complex although the VP/SI transmits it to other brain structures. In contrast motor activity is sensitive to manipulations with both ventral striatal dopamine and rostral VP/SI complex GABA.