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. 2003 May;69(5):2999-3001.
doi: 10.1128/AEM.69.5.2999-3001.2003.

Reductive dehalogenation of chlorobenzene congeners in cell extracts of Dehalococcoides sp. strain CBDB1

Tina Hölscher  1 , Helmut Görisch, Lorenz Adrian
Affiliations

Reductive dehalogenation of chlorobenzene congeners in cell extracts of Dehalococcoides sp. strain CBDB1

Tina Hölscher et al. Appl Environ Microbiol. 2003 May.

Abstract

Enzymatic reductive dehalogenation of tri-, tetra-, penta-, and hexachlorobenzenes was demonstrated in cell extracts with low protein concentration (0.5 to 1 micro g of protein/ml) derived from the chlorobenzene-respiring anaerobe Dehalococcoides sp. strain CBDB1. 1,2,3-trichlorobenzene dehalogenase activity was associated with the membrane fraction. Light-reversible inhibition by alkyl iodides indicated the presence of a corrinoid cofactor.

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Figures

FIG. 1.
FIG. 1.
Product formation from PeCB by cell extracts of strain CBDB1. PeCB, open triangles; 1,2,4,5-TeCB, solid triangles; 1,2,3,5-TeCB, open circles; 1,3,5-TCB, solid circles. Values are means of results of triplicate assays ± standard deviation.
FIG. 2.
FIG. 2.
Aerotolerance and stability of TCB dehalogenase activity. Crude extracts were stored at 4°C under reduced anaerobic conditions (Eh < −200 mV) (triangles), anoxic conditions (Eh ≥ 0 V) (open circles), or aerobic conditions (solid circles). Values are means of results of triplicate assays ± standard deviation. sp act, specific activity.
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References

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