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Review
. 2003 May 13;100(10):5586-8.
doi: 10.1073/pnas.1031802100. Epub 2003 May 5.

Integration by design

Suzanne Sandmeyer  1
Affiliations
Review

Integration by design

Suzanne Sandmeyer. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Figure 1
Figure 1
Strategy for retargeting Ty5 integration. Top, schematic of Ty5 single ORF encoding RNA binding (RB), protease (PR), integrase (IN), reverse transcriptase (RT), and marker gene (his3AI) (open box). View of IN is expanded to show conserved residues and targeting domain (TD) (solid). Lower left, preintegration complex showing wild-type IN bound to ends of Ty5 DNA (thick line) and integrating into telomeric heterochromatin, mediated by Sir4p (hatched). Lower right, same as left except that the natural TD is replaced with heterologous domains (TD*) from Rad9p and NpwBP (solid). LexA DNA-binding domain (open) is expressed fused to Sir4pC, Rad53p FHA1, and Npw38 WW domains (hatched). Integration occurs proximal to LexA-binding sites (open arrows) in plasmid target (closed circle).
See this image and copyright information in PMC

Comment on

References

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