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. 2003 Jun;77(11):6541-5.
doi: 10.1128/jvi.77.11.6541-6545.2003.

Excretion of wild-type and vaccine-derived poliovirus in the feces of poliovirus receptor-transgenic mice

Hein J Boot  1 , Daniella T J Kasteel, Anne-Marie Buisman, Tjeerd G Kimman
Affiliations

Excretion of wild-type and vaccine-derived poliovirus in the feces of poliovirus receptor-transgenic mice

Hein J Boot et al. J Virol. 2003 Jun.

Abstract

The emergence of circulating vaccine-derived poliovirus (cVDPV) strains in suboptimally vaccinated populations is a serious threat to the global poliovirus eradication. The genetic determinants for the transmissibility phenotype of polioviruses, and in particularly of cVDPV strains, are currently unknown. Here we describe the fecal excretion of wild-type poliovirus, oral polio vaccine, and cVDPV (Hispaniola) strains after intraperitoneal injection in poliovirus receptor-transgenic mice. Both the pattern and the level of fecal excretion of the cVDPV strains resemble those of wild-type poliovirus type 1. In contrast, very little poliovirus was present in the feces after oral polio vaccine administration. This mouse model will be helpful in elucidating the genetic determinants for the high fecal-oral transmission phenotype of cVDPV strains.

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Figures

FIG. 1.
FIG. 1.
The group average TCID50 of poliovirus in the feces of individual mice (six ICR-PVRTg21 mice per group) was determined at days 1 to 10 following intraperitoneal injection of different strains and concentrations of poliovirus. Data of representative experiments are shown. (a) Wild-type serotype 1 (Mahoney) was given at a TCID50 of 104, 105, and 106. (b) Intermediate doses (TCID50 = 105) of the three different serotypes of wild-type poliovirus strains. (c) Intermediate doses (TCID50 = 105) of three serotype 1 strains: a vaccine (OPV-1) strain, a wild-type (Mahoney) strain, and a cVDPV (DOR00-016) strain. The detection level of poliovirus in the individual fecal samples is at a TCID50 of 101.8, and the error bars represent the standard errors of the means.
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References

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