Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003;94(6):947-52.
doi: 10.1046/j.1365-2672.2003.01853.x.

Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans

A S Carvalho  1 , J Silva, P Ho, P Teixeira, F X Malcata, P Gibbs
Affiliations
Comparative Study

Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans

A S Carvalho et al. J Appl Microbiol. 2003.

Abstract

Aims: The effects of three different growth media (MRS, M17 and Lee's) on survival during freeze-drying and subsequent storage of six strains of Enterococcus faecalis and two strains of E. durans were investigated.

Methods and results: Distinct Enterococcus spp. strains were grown on M17, MRS and Lee's broth, freeze-dried and stored at 20 degrees C in air under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and then plated on M17 agar.

Conclusions: A higher survival rate during storage of dried E. durans was obtained when growth occurred in MRS. The same effect was not observed, however, for the majority of E. faecalis strains, which clearly survived better in the dried state when this organism had been grown in M17 or Lee's medium.

Significance and impact of study: The survival of the dried Enterococcus spp. tested during storage was shown to be strain-specific and dependent on the growth medium.

PubMed Disclaimer

Publication types

MeSH terms

Substances