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. 2003 Jun;185(11):3476-9.
doi: 10.1128/JB.185.11.3476-3479.2003.

The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA)

Gerald L Newton  1 , Teresa Koledin, Batia Gorovitz, Mamta Rawat, Robert C Fahey, Yossef Av-Gay
Affiliations

The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA)

Gerald L Newton et al. J Bacteriol. 2003 Jun.

Abstract

Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.

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Figures

FIG. 1.
FIG. 1.
Structure and biosynthetic pathway for MSH. M. tuberculosis genes encoding MshB (Rv1170; GlcNAc-Ins deacetylase) (14), MshC (Rv2130c; ATP-dependent l-Cys:GlcN-Ins ligase) (18), and MshD (Rv0819; acetyltransferase, mycothiol synthase) (9) have been identified.
FIG. 2.
FIG. 2.
Alignment of the sequences of MshA from M. smegmatis (MshA-Msmeg) and M. tuberculosis (MshA-Mtub) with the sequences of SpsA from Anaebaena sp. strain PCC7120 (SpsA-Anab) and PimB (Rv0557) from M. tuberculosis (PimB-Mtub). The site of the G32D mutation in M. smegmatis mutant 49 is indicated by an asterisk. See the text for a discussion of specifically designated residues and domains; the numbers are numbers in the M. tuberculosis MshA sequence.
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