doi: 10.1038/nbt830.
Epub 2003 May 18.
Production of knockout rats using ENU mutagenesis and a yeast-based screening assay
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- PMID: 12754522
- DOI: 10.1038/nbt830
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Production of knockout rats using ENU mutagenesis and a yeast-based screening assay
Nat Biotechnol.
2003 Jun.
Abstract
The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.
Comment in
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Targeting mammalian genes--rats join in and mice move ahead.Nat Biotechnol. 2003 Jun;21(6):625-7. doi: 10.1038/nbt0603-625. Nat Biotechnol. 2003. PMID: 12776146 No abstract available.
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