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. 2003 Jun 9;110(1):9-17.
doi: 10.1016/s0166-0934(03)00081-8.

Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy

Annamaria Pratelli  1 , Vito Martella, Nicola Decaro, Antonella Tinelli, Michele Camero, Francesco Cirone, Gabriella Elia, Alessandra Cavalli, Marialaura Corrente, Grazia Greco, Domenico Buonavoglia, Mattia Gentile, Maria Tempesta, Canio Buonavoglia
Affiliations

Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy

Annamaria Pratelli et al. J Virol Methods. .

Abstract

The sequence of the S gene of a field canine coronavirus (CCoV), strain Elmo/02, revealed low nucleotide (61%) and amino acid (54%) identity to reference CCoV strains. The highest correlation (77% nt and 81.7% aa) was found with feline coronavirus type I. A PCR assay for the S gene of strain Elmo/02 detected analogous CCoVs of different geographic origin, all which exhibited at least 92-96% nucleotide identity to each other and to strain Elmo/02. The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population.

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Figures

Fig. 1
Fig. 1
Outlines of the strategy followed to determine the sequence of ORF2 of strain Elmo/02. Dashed arrows indicate the degenerate primers. The position of the other primer pair used in this study, V3F–V3R, is also reported.
Fig. 2
Fig. 2
Alignment of the deduced amino acid sequence of the S protein of the FCoV-like strain Elmo/02 with reference CCoVs (Insavc-1, K378 and 5821), FCoVs type I (UCD1, Black and KU-2), FCoVs type II (79-1146 and 79-1683), TGEVs (Purdue and Miller), PRCoV (RM4), PEDV (CV777) and HCoV-229 (vNotI-tk). The potential glycosilation sites (*) and the putative cleavage site (▾) are indicated.
Fig. 2
Fig. 2
Alignment of the deduced amino acid sequence of the S protein of the FCoV-like strain Elmo/02 with reference CCoVs (Insavc-1, K378 and 5821), FCoVs type I (UCD1, Black and KU-2), FCoVs type II (79-1146 and 79-1683), TGEVs (Purdue and Miller), PRCoV (RM4), PEDV (CV777) and HCoV-229 (vNotI-tk). The potential glycosilation sites (*) and the putative cleavage site (▾) are indicated.
Fig. 3
Fig. 3
Parsimony phylogenetic tree of the S protein of group I coronaviruses. The tree is drawn to scale and rooted using the vaccinal strain vNot-tk of HCoV-229. Sequence for the coronavirus sequences reported is available from Gen Bank under the following accession numbers. CCoVs: Insavc-1, D13096; K378, X77047; C54, A22886; 5821, AB017789. FCoVs type II: 79-1146, X06170; 79-1683, X80799. FCoVs type I: KU-2: D32044; Black, AB088223; UCD1, AB088222. TGEV: Miller, S51223; Purdue, X05695. PRCoV: RM4, Z24675. PEDV: CV777, NC_00346. HCoV: vNotI-tk, NC_002645.1.
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