Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Jun 9;110(1):9-17.
doi: 10.1016/s0166-0934(03)00081-8.

Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy

Annamaria Pratelli  1 , Vito Martella, Nicola Decaro, Antonella Tinelli, Michele Camero, Francesco Cirone, Gabriella Elia, Alessandra Cavalli, Marialaura Corrente, Grazia Greco, Domenico Buonavoglia, Mattia Gentile, Maria Tempesta, Canio Buonavoglia
Affiliations

Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy

Annamaria Pratelli et al. J Virol Methods. .

Abstract

The sequence of the S gene of a field canine coronavirus (CCoV), strain Elmo/02, revealed low nucleotide (61%) and amino acid (54%) identity to reference CCoV strains. The highest correlation (77% nt and 81.7% aa) was found with feline coronavirus type I. A PCR assay for the S gene of strain Elmo/02 detected analogous CCoVs of different geographic origin, all which exhibited at least 92-96% nucleotide identity to each other and to strain Elmo/02. The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Outlines of the strategy followed to determine the sequence of ORF2 of strain Elmo/02. Dashed arrows indicate the degenerate primers. The position of the other primer pair used in this study, V3F–V3R, is also reported.
Fig. 2
Fig. 2
Alignment of the deduced amino acid sequence of the S protein of the FCoV-like strain Elmo/02 with reference CCoVs (Insavc-1, K378 and 5821), FCoVs type I (UCD1, Black and KU-2), FCoVs type II (79-1146 and 79-1683), TGEVs (Purdue and Miller), PRCoV (RM4), PEDV (CV777) and HCoV-229 (vNotI-tk). The potential glycosilation sites (*) and the putative cleavage site (▾) are indicated.
Fig. 2
Fig. 2
Alignment of the deduced amino acid sequence of the S protein of the FCoV-like strain Elmo/02 with reference CCoVs (Insavc-1, K378 and 5821), FCoVs type I (UCD1, Black and KU-2), FCoVs type II (79-1146 and 79-1683), TGEVs (Purdue and Miller), PRCoV (RM4), PEDV (CV777) and HCoV-229 (vNotI-tk). The potential glycosilation sites (*) and the putative cleavage site (▾) are indicated.
Fig. 3
Fig. 3
Parsimony phylogenetic tree of the S protein of group I coronaviruses. The tree is drawn to scale and rooted using the vaccinal strain vNot-tk of HCoV-229. Sequence for the coronavirus sequences reported is available from Gen Bank under the following accession numbers. CCoVs: Insavc-1, D13096; K378, X77047; C54, A22886; 5821, AB017789. FCoVs type II: 79-1146, X06170; 79-1683, X80799. FCoVs type I: KU-2: D32044; Black, AB088223; UCD1, AB088222. TGEV: Miller, S51223; Purdue, X05695. PRCoV: RM4, Z24675. PEDV: CV777, NC_00346. HCoV: vNotI-tk, NC_002645.1.
See this image and copyright information in PMC

References

    1. Buonavoglia, C., Marsilio, F., Cavalli, A., Tiscar, P.G., 1994. L'infezione da coronavirus del cane: indagine sulla presenza del virus in Italia. Not. Farm. Vet. Nr. 2/94, ed. SCIVAC.
    1. Enjuanes L., Brian D., Cavanagh D., Holmes K., Lai M.M.C., Laude H., Masters P., Rottier P., Siddell S., Spaan W.J.M., Taguchi F., Talbot P. Coronaviridae. In: van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeoch D.J., Pringle C.R., Wickner R.B., editors. Virus Taxonomy, Classification and Nomenclature of Viruses. Academic Press; New York: 2000. pp. 835–849.
    1. Hall T.A. bioedit: a user-friendly biological sequence alignment and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 1999;41:95–98.
    1. Herrewegh A.A.P.M., Smeenk I., Horzinek M.C., Rottier P.J.M., de Groot R.J. Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus. J. Virol. 1998;72:4508–4514. - PMC - PubMed
    1. Hingley S.T., Leparc-Goffart I., Weiss R.S. The spike protein of murine coronavirus mouse hepatitis virus strain A59 is not cleaved in primary glial cells and primary hepatocytes. J. Virol. 1998;72:1606–1609. - PMC - PubMed

Publication types

MeSH terms

Substances

Associated data