[Isolation of phospholipase D from cotton seeds]
[Article in
Russian]
- PMID: 1276267
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[Isolation of phospholipase D from cotton seeds]
[Article in
Russian]
Biokhimiia.
1976 Feb.
Abstract
A method of isolating homogenous phospholipase D from cotton seeds is described. The homogeneity of the enzyme preparation is demonstrated by means of polyacrylamide disc electrophoresis, gel filtration through Sephadex G-200 and determination of N-terminal amino acid residue, which turned to be glutamic acid. Molecular weight of the enzyme is found to be 71000 +/- 3000. Several equilibrium forms of the enzyme are observed.
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