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. 2003 Jun;49(6 Pt 1):953-5.
doi: 10.1373/49.6.953.

Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS)

Leo L M Poon  1 , On Kei Wong, Kwok Hung Chan, Winsie Luk, Kwok Yung Yuen, Joseph S M Peiris, Yi Guan
Affiliations

Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS)

Leo L M Poon et al. Clin Chem. 2003 Jun.

Erratum in

  • Clin Chem. 2003 Jul;49(7):1234
No abstract available

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Figures

Figure 1.
Figure 1.
Detection of a novel human coronavirus associated with SARS by real-time quantitative PCR (a), and melting curve analysis of PCR products from clinical samples (b). (a), amplification plot of fluorescence intensity against the PCR cycle. The copy numbers of input plasmid DNA in the reactions are indicated. The x axis denotes the cycle number of a quantitative PCR assay, and the y axis denotes the fluorescence intensity (F1) over the background. (b), melting curve analysis of PCR products from clinical samples. Signals from positive (+ve) samples, negative (−ve) samples, and a water control (water) are indicated. The x axis denotes the temperature (°C), and the y axis denotes the fluorescence intensity (F1) over the background.
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References

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    1. Peiris JSM, Lai ST, Poon LLM, Guan Y, Yam LYC, Lim W, et al. A coronavirus is associated with severe acute respiratory syndrome (SARS). Lancet 2003; (Published online April 8, 2003:http://www.thelancet.com/journal/vol361/iss9365/full/llan.361.9365.early...)..
    1. McIntosh K. Coronaviruses: a comparative review. Curr Top Microbiol Immunol 1974;63:85-112.

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