Comparative Study
Organ culture of normal and carcinogen-treated rat bladder
- PMID: 1277161
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Comparative Study
Organ culture of normal and carcinogen-treated rat bladder
Cancer Res.
1976 Jul.
Abstract
A system for organ culture of rat bladder in defined medium, without serum, is described. Transitional epithelial morphology of normal bladder was well maintained in Waymouth's Medium MB 752/1, while Ham's Medium F12 caused marked epithelial hyperplasia. Hyperplastic epithelial states induced by in vivo administration of the carcinogen, N-methyl-N-nitrosourea, were well maintained in vitro during organ culture in Medium MB 752/1. The combination of hydrocortisone and insulin markedly inhibited the proliferative response of normal bladder epithelium grown in Medium F12.
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