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. 2002 Mar;27(3):194-6.

[Determination of trigonelline in Trigonella foenum-graecum by HPLC]

[Article in Chinese]
Huai-qing Zhao  1 , Yan Qu, Xue-ya Wang, Hong-jian Zhang, Fa-mei Li, Hattori Masao
Affiliations
  • PMID: 12774399

[Determination of trigonelline in Trigonella foenum-graecum by HPLC]

[Article in Chinese]
Huai-qing Zhao et al. Zhongguo Zhong Yao Za Zhi. 2002 Mar.

Abstract

Objective: A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum.

Method: The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm.

Result: The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively.

Conclusion: It provides scientific indexes for quality control of T. foenum-graecum.

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