doi: 10.1016/s0167-7012(03)00034-4.
A microtiter-plate screening method for biofilm disinfection and removal
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- PMID: 12782382
- DOI: 10.1016/s0167-7012(03)00034-4
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A microtiter-plate screening method for biofilm disinfection and removal
J Microbiol Methods.
2003 Aug.
Abstract
A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm.
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