Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Oct;29(10):1782-9.
doi: 10.1007/s00134-003-1789-9. Epub 2003 May 29.

Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Cristiane Ritter  1 , Michael Andrades, Mário Luis C Frota Júnior, Fernanda Bonatto, Ricardo A Pinho, Manuela Polydoro, Fábio Klamt, Cleovaldo T S Pinheiro, Sérgio S Menna-Barreto, José Cláudio F Moreira, Felipe Dal-Pizzol
Affiliations

Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

Cristiane Ritter et al. Intensive Care Med. 2003 Oct.

Abstract

Objective: This study assessed parameters of free radical damage to biomolecules, mitochondrial superoxide production, superoxide dismutase, and catalase activities and their relationship to sepsis mortality.

Design and setting: Prospective animal study in a university laboratory for experimental.

Subjects: 140 male Wistar rats.

Interventions: The animals were randomly divided into three groups: sham-operated (n=20), cecal ligation and perforation resuscitated with normal saline (n=40), and cecal ligation and perforation with normal saline plus antibiotics (n=40).

Measurements and results: Blood samples were collected from all animals 3, 12, and 24 h after CLP through a jugular catheter inserted before CLP. Rats were evaluated during 5 days after the intervention. Nonsurvivor animals were grouped according to the duration between sepsis induction and death, and oxidative parameters were compared to survivors and sham-operated. Lipid peroxidation, protein carbonyls, and superoxide dismutase were significantly increased in nonsurvivor septic rats and were predictive of mortality. We demonstrated that there is a different modulation of superoxide dismutase and catalase in nonsurvivors during the course of septic response. There was a marked increase in superoxide dismutase activity without a proportional increase in catalase activity in nonsurvivors.

Conclusions: This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity

PubMed Disclaimer

Comment in

  • Antioxidant therapy in sepsis.
    Albuszies G, Brückner UB. Albuszies G, et al. Intensive Care Med. 2003 Oct;29(10):1632-6. doi: 10.1007/s00134-003-1861-5. Epub 2003 Aug 16. Intensive Care Med. 2003. PMID: 12923614 No abstract available.

References

    1. FASEB J. 1999 Sep;13(12):1637-46 - PubMed
    1. Clin Chem. 1995 Jun;41(6 Pt 1):867-71 - PubMed
    1. Crit Care Med. 2000 Jan;28(1):143-9 - PubMed
    1. Crit Care Med. 2003 Jan;31(1 Suppl):S29-38 - PubMed
    1. Crit Care Med. 1995 Feb;23(2):376-93 - PubMed

Publication types

LinkOut - more resources