Predominance of Chlamydia trachomatis serovars associated with urogenital infections in females in New Delhi, India

Abstract

Screening for Chlamydia trachomatis was done for 280 endocervical swab samples by PCR specific for endogenous plasmid. Age dependency was seen in symptomatic patients, with a high chlamydial prevalence rate (28%) found in younger women. Genotyping by restriction fragment length polymorphism analysis of omp1 PCR-positive samples showed serovars D, E, and F to be the most prevalent.

FIG. 1.

AluI restriction endonuclease digestion of PCR products of C. trachomatis-positive clinical samples visualized by silver staining on polyacrylamide gel electrophoresis. Lanes: M, molecular size marker; 1, standard serovar D; 2, clinical sample showing banding pattern matching that of standard serovar D; 3, standard serovar E; 4, clinical sample showing banding pattern matching that of standard serovar E; 5, standard serovar F; 6, clinical sample showing banding pattern matching that of standard serovar F; 7, standard serovar I; 8, clinical sample showing banding pattern matching that of standard serovar I; 9, uncut amplicon.