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Comparative Study
. 2003 Jun;41(6):2703-5.
doi: 10.1128/JCM.41.6.2703-2705.2003.

Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species

David R Murdoch  1 , Stanley Mirrett, Lizzie J Harrell, Susan M Donabedian, Marcus J Zervos, L Barth Reller
Affiliations
Comparative Study

Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species

David R Murdoch et al. J Clin Microbiol. 2003 Jun.

Abstract

We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin resistance and 96% when testing for high-level streptomycin resistance.

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References

    1. Chen, Y.-S., S. A. Marshall, P. L. Winokur, S. L. Coffman, W. W. Wilkie, P. R. Murray, C. A. Spiegel, M. A. Pfaller, G. V. Doern, and R. N. Jones. 1998. Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci. J. Clin. Microbiol. 36:2996-3001. - PMC - PubMed
    1. Chow, J. W. 2000. Aminoglycoside resistance in enterococci. Clin. Infect. Dis. 31:586-589. - PubMed
    1. Chow, J. W., M. J. Zervos, S. A. Lerner, L. A. Thal, S. M. Donabedian, D. D. Jaworski, S. Tsai, K. J. Shaw, and D. B. Clewell. 1997. A novel gentamicin resistance gene in Enterococcus. Antimicrob. Agents Chemother. 41:511-514. - PMC - PubMed
    1. Clark, N. C., Ø. Olsvik, J. M. Swenson, C. A. Spiegel, and F. C. Tenover. 1999. Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis. Antimicrob. Agents Chemother. 43:157-160. - PMC - PubMed
    1. d'Azevedo, P. A., C. A. Dias, A. L. S. Gonçalves, F. Rowe, and L. M. Teixeira. 2001. Evaluation of an automated system for the identification and antimicrobial susceptibility testing of enterococci. Diagn. Microbiol. Infect. Dis. 40:157-161. - PubMed

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