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. 2003 Jun;41(6):2734-6.
doi: 10.1128/JCM.41.6.2734-2736.2003.

Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis

Chris Helps  1 , Nicky Reeves, Kathy Egan, Pam Howard, Dave Harbour
Affiliations

Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis

Chris Helps et al. J Clin Microbiol. 2003 Jun.

Abstract

Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

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Figures

FIG. 1.
FIG. 1.
Dilution curves for each component of the three-way PCR. Genomic DNA was isolated from a mixture of FHV-infected feline cells and C. felis and was serially diluted in 10-fold steps. After multiplex PCR, the threshold cycle was plotted against the log10 of the dilution. Dilution curves for FHV (a), C. felis (b), and feline 28S rDNA (c) are shown. Each point represents the average ± standard deviation for three PCRs.
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References

    1. Burgesser, K. M., S. Hotaling, A. Schiebel, S. E. Ashbaugh, S. M. Roberts, and J. K. Collins. 1999. Comparison of PCR, virus isolation, and indirect fluorescent antibody staining in the detection of naturally occurring feline herpesvirus infections. J. Vet. Diagn. Investig. 11:122-126. - PubMed
    1. Gaskell, R., and S. Dawson. 1998. Feline respiratory disease, p. 97-102. In C. E. Greene (ed.), Infectious diseases of the dog and cat, 2nd ed. W. B. Saunders, Philadelphia, Pa.
    1. Helps, C., N. Reeves, S. Tasker, and D. Harbour. 2001. Use of real-time quantitative PCR to detect Chlamydophila felis infection. J. Clin. Microbiol. 39:2675-2676. - PMC - PubMed
    1. Hoskins, J. D. 1999. Feline respiratory diseases. Vet. Clin. N. Am. Small Anim. Pract. 29:945-958. - PubMed
    1. Johnson, F. W. 1984. Isolation of Chlamydia psittaci from nasal and conjunctival exudate of a domestic cat. Vet. Rec. 114:342-344. - PubMed

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