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. 2003 Jun;9(6):1318-22.
doi: 10.3748/wjg.v9.i6.1318.

Role of nitric oxide and peroxynitrite anion in lung injury induced by intestinal ischemia-reperfusion in rats

Jun-Lin Zhou  1 , Guo-Hua Jin, Yi-Ling Yi, Jun-Lan Zhang, Xin-Li Huang
Affiliations

Role of nitric oxide and peroxynitrite anion in lung injury induced by intestinal ischemia-reperfusion in rats

Jun-Lin Zhou et al. World J Gastroenterol. 2003 Jun.

Abstract

Aim: To evaluate effects of nitric oxide (NO) and peroxynitrite anion (ONOO(-)) on lung injury following intestinal ischemia-reperfusion (IR) in rats.

Methods: A rat model of intestinal ischemia was made by clamping superior mesenteric artery and lung injury was resulted from reperfusion. The animals were randomly divided into 3 groups: sham operation (Sham), 2 h ischemia followed by 2 h reperfusion (IR) and IR pretreated with aminoguanidine (AG) - an inhibitor of inducible NO synthase (iNOS) 15 minutes before reperfusion (IR+AG). The lung malondialdehyde (MDA) and nitrate/nitrite (NO(2)(-)/NO(3)(-)) contents and morphological changes were examined. Western blot was used to detect the iNOS protein expression. Immunohistochemical staining was used to determine the change of nitrotyrosine (NT)- a specific "footprint" of ONOO(-).

Results: The morphology revealed evidence for lung edema, hemorrhage and polymorphonuclear sequestration after intestinal IR. Compared with sham group, lung contents of MDA and NO(2)(-)/NO(3)(-) in IR group were significantly increased (12.00+/-2.18 vs 23.44+/-1.25 and 76.39+/-6.08 vs 140.40+/-4.34, P<0.01) and the positive signals of iNOS and NT were also increased in the lung. Compared with IR group, the contents of MDA and NO(2)(-)/NO(3)(-) in IR+AG group were significantly decreased (23.44+/-1.25 vs 14.66+/-1.66 and 140.40+/-4.34 vs 80.00+/-8.56, P<0.01) and NT staining was also decreased.

Conclusion: Intestinal IR increases NO and ONOO(-) production in the lung, which may be involved in intestinal IR-mediated lung injury.

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Figures

Figure 1
Figure 1
Light microscopic observation on the lung after IIR with pretreatment of AG in rats (HE × 400). A. The normal lung tissue structure was found in sham group; B. Lung edema, hemorrhage and inflammatory cells sequestration were found in the IR group; C. Decreased morphological changes induced by the intestinal IR were found in the IR + AG group.
Figure 2
Figure 2
Western blotting analysis of iNOS in rat lung after IIR with pretreatment of AG in rats. 1. Sham; 2. IR; 3. IR + AG.
Figure 3
Figure 3
Immunohistochemical analysis of NT in the lung after IIR with pretreatment of AG in rats. SP stain × 400. A. No positive signal was found in the lung in sham group; B. Intense positive NT staining was found in the IR group; C. Positive NT staining decreased in the IR + AG group.
See this image and copyright information in PMC

References

    1. Carden DL, Granger DN. Pathophysiology of ischaemia-reperfusion injury. J Pathol. 2000;190:255–266. - PubMed
    1. Rotstein OD. Pathogenesis of multiple organ dysfunction syndrome: gut origin, protection, and decontamination. Surg Infect (Larchmt) 2000;1:217–223; discussion 223-225. - PubMed
    1. Fu XB, Yang YH, Sun TZ, Gu XM, Jiang LX, Sun XQ, Sheng ZY. Effect of intestinal ischemia-reperfusion on expressions of endogenous basic fibroblast growth factor and transforming growth factor betain lung and its relation with lung repair. World J Gastroenterol. 2000;6:353–355. - PMC - PubMed
    1. Wu XT, Li JS, Zhao XF, Zhuang W, Feng XL. Modified techniques of heterotopic total small intestinal transplantation in rats. World J Gastroenterol. 2002;8:758–762. - PMC - PubMed
    1. Mitsuoka H, Schmid-Schönbein GW. Mechanisms for blockade of in vivo activator production in the ischemic intestine and multi-organ failure. Shock. 2000;14:522–527. - PubMed