Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov 15;288 ( Pt 1)(Pt 1):285-90.
doi: 10.1042/bj2880285.

Molecular identification of the liver- and the heart-type fatty acid-binding proteins in human and rat kidney. Use of the reverse transcriptase polymerase chain reaction

R G Maatman  1 , E M van de Westerlo, T H van Kuppevelt, J H Veerkamp
Affiliations

Molecular identification of the liver- and the heart-type fatty acid-binding proteins in human and rat kidney. Use of the reverse transcriptase polymerase chain reaction

R G Maatman et al. Biochem J. .

Abstract

The cDNAs of two types of fatty acid-binding protein (FABP) present in human kidney, previously described as types A and B, were isolated using reverse transcriptase-PCR (RT-PCR) with human kidney mRNA and various sets of primers. The cDNA fragments were cloned and sequenced. Renal FABP type A and B cDNAs appeared to be completely identical to human liver- and heart-type FABP cDNAs respectively. In the second part of this study we demonstrated the presence of liver-type FABP in rat kidney by chromatography, e.l.i.s.a. and immunocytochemistry. The ratio and cellular distribution of the two FABP types varies markedly in human and rat kidney. Using RT-PCR we were also able to prepare and identify liver- and heart-type FABP cDNAs with mRNA from both male and female rat kidney.

PubMed Disclaimer

References

    1. Anat Rec. 1991 Jan;229(1):51-60 - PubMed
    1. Biochim Biophys Acta. 1991 Jan 4;1081(1):1-24 - PubMed
    1. J Biol Chem. 1991 Mar 25;266(9):5963-72 - PubMed
    1. Int J Biochem. 1990;22(4):393-8 - PubMed
    1. Proc Soc Exp Biol Med. 1986 Sep;182(4):531-9 - PubMed

MeSH terms