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. 2003 Aug;90(6):473-5.
doi: 10.1007/s00436-003-0890-x. Epub 2003 Jun 12.

Amplified fragment length polymorphism analyses of Eimeria spp.: an improved process for genetic studies of recombinant parasites

D P Blake  1 , A L Smith, M W Shirley
Affiliations

Amplified fragment length polymorphism analyses of Eimeria spp.: an improved process for genetic studies of recombinant parasites

D P Blake et al. Parasitol Res. 2003 Aug.

Abstract

Application of the amplified fragment length polymorphism (AFLP) technique to genetic mapping studies requires high quality DNA as a template. In the case of Eimeria spp., this has previously been in the form of chromosomal DNA obtained from purified sporozoites recovered from large numbers of oocysts (generally up to 2 x 10(8)). In order for the AFLP technique to be more easily applied to studies on the genetics of Eimeria maxima, for which only smaller numbers of oocysts are available, a simplified, more efficient method for the recovery of genomic DNA from small numbers of oocysts was developed. Our new method should also be useful for genetic analyses of other coccidial parasites and for the recovery of AFLP-quality DNA from other pathogens.

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References

    1. Nucleic Acids Res. 1995 Nov 11;23(21):4407-14 - PubMed
    1. Mol Gen Genet. 1995 Nov 1;249(1):74-81 - PubMed
    1. Mol Biochem Parasitol. 2002 Aug 28;123(2):95-104 - PubMed
    1. Mol Biochem Parasitol. 1990 Jan 15;38(2):169-73 - PubMed
    1. Int J Syst Evol Microbiol. 2002 Mar;52(Pt 2):573-86 - PubMed

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