Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification
- PMID: 1280641
- PMCID: PMC270525
- DOI: 10.1128/jcm.30.11.2772-2775.1992
Genetic heterogeneity within Mycobacterium fortuitum complex species: genotypic criteria for identification
Abstract
A 1.5-kb segment of the DNA that encodes 16S rRNA was amplified by polymerase chain reaction, and 880 nucleotide positions were determined from each of the described biovariants of Mycobacterium fortuitum. Signature sequences which allow rapid identification of M. fortuitum strains at the biovariant level are described. Our data demonstrate a close phylogenetic relationship between Mycobacterium senegalense and M. fortuitum and indicate that the described biovariants of M. fortuitum represent genetically distinct taxa.
Similar articles
-
Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1653-1667. doi: 10.1099/ijs.0.02743-0. Int J Syst Evol Microbiol. 2004. PMID: 15388725
-
Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.J Clin Microbiol. 1996 Apr;34(4):866-9. doi: 10.1128/jcm.34.4.866-869.1996. J Clin Microbiol. 1996. PMID: 8815098 Free PMC article.
-
Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing.Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2095-2105. doi: 10.1099/ijs.0.63094-0. Int J Syst Evol Microbiol. 2004. PMID: 15545441
-
Molecular detection of nontuberculous mycobacteria: advantages and limits of a broad-range sequencing approach.J Mol Microbiol Biotechnol. 2012;22(4):268-76. doi: 10.1159/000342517. Epub 2012 Oct 4. J Mol Microbiol Biotechnol. 2012. PMID: 23037303 Review.
-
Nontuberculous mycobacteria: Insights on taxonomy and evolution.Infect Genet Evol. 2019 Aug;72:159-168. doi: 10.1016/j.meegid.2019.01.017. Epub 2019 Jan 14. Infect Genet Evol. 2019. PMID: 30654178 Review.
Cited by
-
PCR-enzyme-linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria.J Clin Microbiol. 1997 Sep;35(9):2375-80. doi: 10.1128/jcm.35.9.2375-2380.1997. J Clin Microbiol. 1997. PMID: 9276419 Free PMC article.
-
hsp65 sequencing for identification of rapidly growing mycobacteria.J Clin Microbiol. 1999 Mar;37(3):852-7. doi: 10.1128/JCM.37.3.852-857.1999. J Clin Microbiol. 1999. PMID: 9986875 Free PMC article.
-
Clinical and taxonomic status of pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria.Clin Microbiol Rev. 2002 Oct;15(4):716-46. doi: 10.1128/CMR.15.4.716-746.2002. Clin Microbiol Rev. 2002. PMID: 12364376 Free PMC article. Review.
-
Mycobacterial taxonomy.Eur J Clin Microbiol Infect Dis. 1994 Nov;13(11):884-901. doi: 10.1007/BF02111489. Eur J Clin Microbiol Infect Dis. 1994. PMID: 7698114 Review.
-
Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis.J Clin Microbiol. 1993 Aug;31(8):2061-5. doi: 10.1128/jcm.31.8.2061-2065.1993. J Clin Microbiol. 1993. PMID: 8396586 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases