Multilocus enzyme electrophoresis of major O-antigen reference strains of Pseudomonas aeruginosa

Abstract

Multilocus enzyme electrophoresis was used to characterize 27 Pseudomonas aeruginosa serogroup reference strains used in the major O-antigen schemes according to which Pseudomonas aeruginosa has been typed. Sixteen enzyme loci were assayed, ten of which showed electrophoretic variation. Genetic diversity was expressed for each enzyme locus, and as the mean allelic diversity of loci. Ten electrophoretic types were identified among the strains. The genetic distance between pairs of electrophoretic types was expressed as the proportion of loci at which similar alleles occurred. More than 80% similarity was observed between any pair of electrophoretic types, reflecting the homogeneity of multilocus genotypes within this species. Similarity between electrophoretic types was represented in the form of a dendrogram and by multi-dimensional scaling. Three distinct clusters of electrophoretic types were revealed; within each the serogroups appeared to be randomly distributed.