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. 1992 Feb;1(3):160-3.
doi: 10.1101/gr.1.3.160.

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide

J Singer-Sam  1 , J M LeBon, A Dai, A D Riggs
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Free article

A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide

J Singer-Sam et al. PCR Methods Appl. 1992 Feb.
Free article

Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

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