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. 2003 Jul;47(7):2223-30.
doi: 10.1128/AAC.47.7.2223-2230.2003.

Detection of inhibition of bovine viral diarrhea virus by aromatic cationic molecules

M Daniel Givens  1 , Christine C Dykstra, Kenny V Brock, David A Stringfellow, Arvind Kumar, Chad E Stephens, Hakan Goker, David W Boykin
Affiliations

Detection of inhibition of bovine viral diarrhea virus by aromatic cationic molecules

M Daniel Givens et al. Antimicrob Agents Chemother. 2003 Jul.

Abstract

Bovine viral diarrhea virus (BVDV) is an economically significant pathogen of cattle and a problematic contaminant in the laboratory. BVDV is often used as an in vitro model for hepatitis C virus during drug discovery efforts. Aromatic dicationic molecules have exhibited inhibitory activity against several RNA viruses. Thus, the purpose of this research was to develop and apply a method for screening the aromatic cationic compounds for in vitro cytotoxicity and activity against a noncytopathic strain of BVDV. The screening method evaluated the concentration of BVDV in medium and cell lysates after 72 h of cell culture in the presence of either a 25 or 5 microM concentration of the test compound. Five of 93 screened compounds were selected for further determination of inhibitory (90 and 50%) and cytotoxic (50 and 10%) concentration endpoints. The screening method identified compounds that exhibited inhibition of BVDV at nanomolar concentrations while exhibiting no cytotoxicity at 25 microM concentrations. The leading compounds require further investigation to determine their mechanism of action, in vivo activity, and specific activity against hepatitis C virus.

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Figures

FIG. 1.
FIG. 1.
Curve fit analysis for cytotoxic concentrations and BVDV inhibitory concentrations in cell culture of selected aromatic cationic molecules.
See this image and copyright information in PMC

References

    1. Afshar, A., G. C. Dulac, C. Dubuc, and T. H. Howard. 1991. Comparative evaluation of the fluorescent antibody test and microtiter immunoperoxidase assay for detection of bovine viral diarrhea virus from bull semen. Can. J. Vet. Res. 55:91-93. - PMC - PubMed
    1. Baginski, S. G., D. C. Pevear, M. Seipel, S. C. Sun, C. A. Benetatos, S. K. Chunduru, C. M. Rice, and M. S. Collett. 2000. Mechanism of action of a pestivirus antiviral compound. Proc. Natl. Acad. Sci. USA 97:7981-7986. - PMC - PubMed
    1. Baker, J. C. 1987. Bovine viral diarrhea virus: a review. J. Am. Vet. Med. Assoc. 190:1449-1458. - PubMed
    1. Behrens, S.-E., C. W. Grassmann, H.-J. Thiel, G. Meyers, and N. Tautz. 1998. Characterization of an autonomous subgenomic pestivirus RNA replicon. J. Virol. 72:2364-2372. - PMC - PubMed
    1. Blight, K. J., A. A. Kolykhalov, and C. M. Rice. 2000. Efficient initiation of HCV RNA replication in cell culture. Science 290:1972-1974. - PubMed

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