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. 2003 Jul;47(7):2307-9.
doi: 10.1128/AAC.47.7.2307-2309.2003.

Nonsense mutations in the lsa-like gene in Enterococcus faecalis isolates susceptible to lincosamides and Streptogramins A

Julia Dina  1 , Brigitte Malbruny, Roland Leclercq
Affiliations

Nonsense mutations in the lsa-like gene in Enterococcus faecalis isolates susceptible to lincosamides and Streptogramins A

Julia Dina et al. Antimicrob Agents Chemother. 2003 Jul.

Abstract

The lsa gene confers intrinsic resistance to lincosamides and streptogramins A in Enterococcus faecalis, probably by active efflux. The lsa-like genes of two clinical isolates of E. faecalis susceptible to lincosamides and dalfopristin contained mutations that produced premature termination codons. Revertant mutants were obtained by selection on agar plates containing clindamycin.

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Figures

FIG. 1.
FIG. 1.
Multiple-sequence alignment of deduced amino acid sequences of the lsa-like genes from E. faecalis V583, ATCC 29212, UCN32, and UCN33. ClustalW at the Infobiogen website (http://www.infobiogen.fr) was used. The two ATP-binding domains consisting of Walker A (WA) and Walker B (WB) motifs (marked in solid black) and of a signature (SGG) sequence (underlined) are shown. Asterisks indicate differences in the amino acids.
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