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. 2003 Apr;22(2):87-90.
doi: 10.1089/153685903321948003.

Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit

W Nastainczyk  1 , O-G Issinger, B Guerra
Affiliations

Epitope analysis of the MAb 1AD9 antibody detection site in human protein kinase CK2alpha-subunit

W Nastainczyk et al. Hybrid Hybridomics. 2003 Apr.

Abstract

1AD9 is a murine monoclonal antibody (MAb) that was produced against the recombinant human alpha-subunit of protein kinase CK2, a pleiotropic and constitutively active serine/threonine protein kinase. We have further characterized this antibody, which is suitable for Western blot, immunoprecipitation and enzyme-linked immunosorbant assay tests. Using an overlapping peptide library, we have identified the epitope targeted by MAb1AD9 characterized by the following sequence: (319)MEHPYF(324).

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