Projections to the inferior colliculus from the dorsal column nuclei. An experimental electron microscopic study in the cat

Abstract

The projections from the dorsal column nuclei (DCN) to the inferior colliculus (IC) were investigated in cats by means of electron microscopy. The DCN were destroyed unilaterally by electrocoagulation and the animals survived 3 or 4 days. A variable number of degenerating synaptic boutons were observed bilaterally in the IC-external nucleus and in the intercollicular area. The greatest number of degenerating terminals was encountered in the rostral portions of the IC-external nucleus and in the intercollicular area contralateral to the DCN lesion. In these regions some series of ultrathin sections the degenerating boutons comprised 6-13% of the entire synaptic bouton population of the examined area. In the caudal portion of the contralateral IC-external nucleus the number of degenerating boutons greatly diminished and the latter showed a patchy distribution. Ipsilateral to the DCN lesion the number of degenerating terminals was low, and a more substantial number was found only in the rostroventral portion of the IC-external nucleus and in the intercollicular area. The degenerating synaptic boutons displayed markedly diverse patterns of alterations. Most often was the dark (electron-dense) degeneration pattern, followed by the filamentous degeneration. More rare were the light (electron-lucent) degenerating boutons, and a very limited number of terminals displayed pinocytotic degeneration changes. The DCN boutons contained round and oval synaptic vesicles and terminated mainly on proximal dendritic trunks, followed by the perikarya of the efferent neurons, and smaller, distal dendrites. Rarely the degenerating terminals contacted dendritic spines, and no contacts were found with the small (interneuronal) perikarya and with other vesicle containing profiles. The axodendritic contacts were asymmetrical and the axosomatic--from the intermediate type. In agreement with previous light microscopic data, the present findings demonstrate the existence of substantial excitatory projection from the DCN to the polysensory nuclei of the IC that integrate converging auditory and tactile information, and are involved in acoustico-motor behavior.