Surface plasmon resonance mass spectrometry: recent progress and outlooks
- PMID: 12837614
- DOI: 10.1016/S0167-7799(03)00141-0
Surface plasmon resonance mass spectrometry: recent progress and outlooks
Abstract
The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) has created a unique approach to protein investigations. Surface plasmon resonance is used to quantify interactions between proteins and surface-immobilized ligands, and MS is used to determine the structural features of the bound proteins. Recent progress in SPR-MS includes improved methods and operations, increased limits of detection, multi-protein analysis and protein-complex delineation. With the subsequent design of SPR protein arrays, SPR-MS is expected to enter into the field of high-throughput protein interaction discovery and miniaturized diagnostics.
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