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. 2003 Jul;69(7):4332-6.
doi: 10.1128/AEM.69.7.4332-4336.2003.

Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles

Dhritiman Ghosh  1 , Bijay Bal, V K Kashyap, Subrata Pal
Affiliations

Molecular phylogenetic exploration of bacterial diversity in a Bakreshwar (India) hot spring and culture of Shewanella-related thermophiles

Dhritiman Ghosh et al. Appl Environ Microbiol. 2003 Jul.

Abstract

The bacterial diversity of a hot spring in Bakreshwar, India, was investigated by a culture-independent approach. 16S ribosomal DNA clones derived from the sediment samples were found to be associated with gamma-Proteobacteria, cyanobacteria, and green nonsulfur and low-GC gram-positive bacteria. The first of the above phylotypes cobranches with Shewanella, a well-known iron reducer. This phylogenetic correlation has been exploited to develop culture conditions for thermophilic iron-reducing microorganisms.

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Figures

FIG. 1.
FIG. 1.
Phylogenetic tree deduced from the gamma-proteobacterial 16S rDNA of hot spring sediment clones by maximum likelihood algorithm. Reference sequences were chosen to represent the broadest diversity of Bacteria. Aquifex pyrophilus and Chloroflexus aggregans were used as outgroups for the analysis. Division- and subdivision-level groupings are either bracketed or marked with a horizontal line at the right of the figure. Branch points supported (bootstrap values) are indicated at the branch points of the nodes of the tree. A scale is shown below the tree to indicate the branch length.
FIG. 2.
FIG. 2.
Growth of Shewanella-like consortium at 66°C and Fe(III) reduction in a medium with pyruvate as the electron donor and poorly crystalline Fe(III) oxide as the electron acceptor. The medium also contained 0.01% yeast extract. The results are the means of triplicate cultures. OD540, optical density at 540 nm.
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