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. 2003 Jul;41(7):3312-6.
doi: 10.1128/JCM.41.7.3312-3316.2003.

Novel selective medium for isolation of Burkholderia pseudomallei

K Howard  1 , T J J Inglis
Affiliations

Novel selective medium for isolation of Burkholderia pseudomallei

K Howard et al. J Clin Microbiol. 2003 Jul.

Abstract

Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas aeruginosa were used to determine the selectivity and sensitivity of BPSA. BPSA was more inhibitory to P. aeruginosa and B. cepacia and should make recognition of Burkholderia species easier due to distinctive colony morphology. BPSA also inhibited Enterococcus, Escherichia, Staphylococcus, and Streptococcus: These results indicate that BPSA is a potential replacement for ASA.

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Figures

FIG. 1.
FIG. 1.
Colony counts of B. pseudomallei strains after 48 h of incubation on ASA (white bars) and BPSA (gray bars).
FIG.2.
FIG.2.
Colony morphology at 48 h (left) and 7 days (right) of B. pseudomallei grown on BPSA. (a and d) Strain NCTC 13177; (b and e) strain NCTC 10276; (c and f) the persistently mucoid strain WACC11. Bar = 1 mm.
See this image and copyright information in PMC

References

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